Project 1

New animal cloning technology

Team

Professor Pengtao Liu

HKU
Project Leader

Professor William Yeung

HKU
Co-Pls & Collaborators

Dr. Cherie Lee

HKU
Co-Pls & Collaborators

Project Summary

A new animal cloning technology. From one EPSC to a mouse: Self- assembling of EPSCs to blastoids

EPSCs re-build embryo: Blastoid

Develop novel animal cloning technologies in translational stem cell research and map cell lineage from human EPSCs to cell types relevant to regenerative medicine and immunotherapies, as to optimize protocols for efficient generation of specific cell types from human EPSCs

Human EPSCs retain developmental potential to all cell types including both embryonic somatic and germline cell lineages and to extra-embryonic trophoblasts in the placenta. With TGF-b inhibition, human EPSCs can differentiate to trophoblasts, which can be infected with SARS-CoV-2. EPSC-derived trophoblast stem cells (TSCs) show bidirected potential, which can further differentiate to subtype cells STBs and EVTs. EPSC-derived TSCs can also generate 3D trophoblast organoids, which recapitulate the physiological arrangements of the human placenta tissue derived trophoblast organoids.