Programme 2

EPSCs for Studying Human Disease and for Regenerative Medicine

Team

Professor Hung Fat Tse

HKU
Leader

Professor Pengtao Liu

HKU
Co-Pls & Collaborators

Professor William Yeung

HKU
Co-Pls & Collaborators

Dr. Cherie Lee

HKU
Co-Pls & Collaborators

Dr. Rio Sugimura

HKU
Co-Pls & Collaborators

Dr. Stephanie Ma

HKU
Co-Pls & Collaborators

Programme Summary



Clinical translation of EPSCs: Clinical grade MSCs can be differentiated from human EPSCs which is easier in culture, and genetically and epigenetically stable, and allow more efficient genome-editing to generate modified MSCs that provide better therapeutic potential and further reduce their immune rejection after transplantation.

Clinical grade human EPSC-MSCs will first undergo extensive pre-clinical evaluation for safety and efficacy for transplantation in our established clinically relevant large animal models of heart diseases.

These clinical grade human EPSC lines and the products will be an important part of the Centre’s propriety technology and platform.

Programme Description

  • Application of stem cell-derived cells for disease modeling/drug screening
  • Cell products
  • EPSCs as platform for the generation of genome-edited animals
Publications

Projects

Publication

  1. Lee YL, Fong SW, Chen AC… Yeung WS, Lee KF. (2015) Establishment of a novel human embryonic stem cell-derived trophoblastic spheroid implantation model. Hum Reprod. 30 (11): 2614-26. http://dx.doi.org/10.1093/humrep/dev223
  2. Yang J, Wang Y, Zhou T… Esteban MA, Tse HF. (2017) Generation of Human Liver Chimeric Mice with Hepatocytes from Familial Hypercholesterolemia Induced Pluripotent Stem Cells. Stem Cell Reports 8: 603-618. https://doi.org/10.1016/j.stemcr.2017.01.027
  3. Sampaziotis F, Justin AW, Tysoe OC… Saeb-Parsy K*, Vallier L*. (2017) Reconstruction of the mouse extrahepatic biliary tree using primary human extrahepatic cholangiocyte organoids. Nature Medicine 23(8): 954-963. (*joint authorship) https://doi.org/10.1038/nm.4360
  4. Segeritz CP, Rashid ST, Cardoso de Brito M… Lomas DA*, Vallier L*. (2018) hiPSC hepatocyte model demonstrates the role of unfolded protein response and inflammatory networks in α1-antitrypsin deficiency. J Hepatol. 69(4): 851-860. (*joint authorship) https://doi.org/10.1016/j.jhep.2018.05.028
  5. Chan LH, Zhou L, Ng KY… Huen MS, Ma S. (2018) Protein arginine methyltransferase PRMT6 regulates RAS/RAF binding and MEK/ERK-mediated cancer stemness activities in hepatocellular carcinoma through CRAF methylation. Cell Rep 25: 690-701. http://dx.doi.org/10.1016/j.celrep.2018.09.053
  6. Yang J, Ryan DJ, Lan G, Zou X, Liu P. (2019) In vitro establishment of expanded-potential stem cells from mouse pre-implantation embryos or embryonic stem cells. Nature Protocols, 14: 350-378. http://dx.doi.org/10.1038/s41596-018-0096-4   
  7. Ohta R*, Sugimura R*, Niwa A, Saito MK. (2019) Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-Free Defined Condition. J Vis Exp JoVE 148. https://doi.org/10.3791/59823 (*co-first author)
  8. Yue C, Chen ACH, Tian S… Yeung WSB, Lee YL. (2020) Human embryonic stem cell–derived blastocyst-like spheroids resemble human trophectoderm during early implantation process. Fertil Steril 114(3): 653-664. https://doi.org/10.1016/j.fertnstert.2020.01.009
  9. Lee TK, Guan X, Ma SKY. (2021) Cancer stem cells in hepatocellular carcinoma: from origin to clinical implications. Nature Reviews Gastroenterology and Hepatology 9. https://doi.org/10.1038/s41575-021-00508-3

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